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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- GTX628138 - Provider product page
- Provider
- GeneTex
- Product name
- Lin28A antibody [GT717]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
Submitted references Over-expression of cofilin-1 suppressed growth and invasion of cancer cells is associated with up-regulation of let-7 microRNA.
Tsai CH, Lin LT, Wang CY, Chiu YW, Chou YT, Chiu SJ, Wang HE, Liu RS, Wu CY, Chan PC, Yang MH, Chiou SH, Liao MJ, Lee YJ
Biochimica et biophysica acta 2015 May;1852(5):851-61
Biochimica et biophysica acta 2015 May;1852(5):851-61
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Lin28A antibody [GT717] detects LIN28 protein by Western blot analysis.A. 30 £gg human ESC whole cell lysate/extract12 % SDS-PAGELin28A antibody [GT717] (GTX628138) dilution: 1:1000
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: NT2D1 12% SDS PAGE GTX628138 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Non-transfected HeLa lysates B: LIN28 transfected HeLa lysates 12% SDS PAGE GTX628138 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 £gg) was separated by 12% SDS-PAGE, and the membrane was blotted with Lin28A antibody [GT717] (GTX628138) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- LIN28 antibody detects LIN28 protein by immunofluorescent analysis. Sample: human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min. Green: LIN28 protein stained by LIN28 antibody (GTX628138) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 £gm.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Lin28A antibody detects Lin28A protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Lin28A antibody (GTX628138) dilution: 1:100.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Lin28A antibody [GT717] detects Lin28A protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Lin28A antibody [GT717] dilution: 1:50
