Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- ABIN953174 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Lin-28 Homolog A (C. Elegans) (LIN28A) (AA 115-147), (Middle Region) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 115-147 amino acids from the Central region of human LIN28A
- Description
- Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Epitope
- AA 115-147,Middle Region
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Lin28-mediated post-transcriptional regulation of Oct4 expression in human embryonic stem cells.
Pluripotency factors Lin28 and Oct4 identify a sub-population of stem cell-like cells in ovarian cancer.
An epigenetic switch involving NF-kappaB, Lin28, Let-7 MicroRNA, and IL6 links inflammation to cell transformation.
TUT4 in concert with Lin28 suppresses microRNA biogenesis through pre-microRNA uridylation.
A role for Lin28 in primordial germ-cell development and germ-cell malignancy.
Qiu C, Ma Y, Wang J, Peng S, Huang Y
Nucleic acids research 2010 Mar;38(4):1240-8
Nucleic acids research 2010 Mar;38(4):1240-8
Pluripotency factors Lin28 and Oct4 identify a sub-population of stem cell-like cells in ovarian cancer.
Peng S, Maihle NJ, Huang Y
Oncogene 2010 Apr 8;29(14):2153-9
Oncogene 2010 Apr 8;29(14):2153-9
An epigenetic switch involving NF-kappaB, Lin28, Let-7 MicroRNA, and IL6 links inflammation to cell transformation.
Iliopoulos D, Hirsch HA, Struhl K
Cell 2009 Nov 13;139(4):693-706
Cell 2009 Nov 13;139(4):693-706
TUT4 in concert with Lin28 suppresses microRNA biogenesis through pre-microRNA uridylation.
Heo I, Joo C, Kim YK, Ha M, Yoon MJ, Cho J, Yeom KH, Han J, Kim VN
Cell 2009 Aug 21;138(4):696-708
Cell 2009 Aug 21;138(4):696-708
A role for Lin28 in primordial germ-cell development and germ-cell malignancy.
West JA, Viswanathan SR, Yabuuchi A, Cunniff K, Takeuchi A, Park IH, Sero JE, Zhu H, Perez-Atayde A, Frazier AL, Surani MA, Daley GQ
Nature 2009 Aug 13;460(7257):909-13
Nature 2009 Aug 13;460(7257):909-13
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of LIN28A Antibody (Center) Cat.-No AP52487PU-N in mouse Neuro-2a cell line lysates (35ug/lane). This demonstrates the LIN28A antibody detected the LIN28A protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue reacted with LIN28A Antibody (Center) Cat.-No AP52487PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells using LIN28A Antibody (Center) Cat.-No AP52487PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.