12316-1-AP

antibody from Invitrogen Antibodies

Targeting: ANGPTL2 ARP2, HARP

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12316-1-AP

Provider

Invitrogen Antibodies

Product name

ANGPTL2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: QSEIIAQLE EHCQRVPSAR PVPQPPPAAP PRVYQPPTYN RIINQISTNE IQSDQNLKVL PPPLPTMPTL TSLPSSTDKP SGPWRDCLQA LEDGHDTSSI YLVKPENTNR LMQVWCDQRH DPGGWTVIQR RLDGSVNFFR NWETYKQGFG NIDGEYWLGL ENIYWLTNQG NYKLLVTMED WSGRKVFAEY ASFRLEPESE YYKLRLGRYH GNAGDSFTWH NGKQFTTLDR DHDVYTGNCA HYQKGGWWYN ACAHSNLNGV WYRGGHYRSR YQDGVYWAEF RGGSYSLKKV VMMIRPNPNT FH (193-493 aa encoded by BC012368)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.43 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 12316-1-AP (ANGPTL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

3T3-L1 cells were subjected to SDS PAGE followed by western blot with 12316-1-AP (ANGPTL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 12316-1-AP (ANGPTL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12316-1-AP (ANGPTL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse heart tissue were subjected to SDS PAGE followed by western blot with 12316-1-AP (ANGPTL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart using 12316-1-AP (ANGPTL2 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart using 12316-1-AP (ANGPTL2 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human spleen using 12316-1-AP (ANGPTL2 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human spleen using 12316-1-AP (ANGPTL2 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 12316-1-AP (ANGPTL2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 12316-1-AP (ANGPTL2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using (Product # 12316-1-AP) (ANGPTL2 antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ANGPTL2 (IP:12316-1-AP, 4ug; Detection:12316-1-AP 1:500) with mouse testis tissue lysate 4800ug.