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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3274 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3274, RRID:AB_1578216
- Product name
- NUAK1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of NUAK1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references ARK5 is a tumor invasion-associated factor downstream of Akt signaling.
ARK5 suppresses the cell death induced by nutrient starvation and death receptors via inhibition of caspase 8 activation, but not by chemotherapeutic agents or UV irradiation.
Identification of a novel protein kinase mediating Akt survival signaling to the ATM protein.
Suzuki A, Lu J, Kusakai G, Kishimoto A, Ogura T, Esumi H
Molecular and cellular biology 2004 Apr;24(8):3526-35
Molecular and cellular biology 2004 Apr;24(8):3526-35
ARK5 suppresses the cell death induced by nutrient starvation and death receptors via inhibition of caspase 8 activation, but not by chemotherapeutic agents or UV irradiation.
Suzuki A, Kusakai G, Kishimoto A, Lu J, Ogura T, Esumi H
Oncogene 2003 Sep 18;22(40):6177-82
Oncogene 2003 Sep 18;22(40):6177-82
Identification of a novel protein kinase mediating Akt survival signaling to the ATM protein.
Suzuki A, Kusakai G, Kishimoto A, Lu J, Ogura T, Lavin MF, Esumi H
The Journal of biological chemistry 2003 Jan 3;278(1):48-53
The Journal of biological chemistry 2003 Jan 3;278(1):48-53
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of NUAK1 polyclonal antibody (Cat # PAB3274) in mouse brain tissue lysate. NUAK1 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with NUAK1 polyclonal antibody (Cat # PAB3274) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
