PA5-80580

antibody from Invitrogen Antibodies

Targeting: EMC8 C16orf2, C16orf4, COX4NB, FAM158B, NOC4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-80580

Provider

Invitrogen Antibodies

Product name

COX4NB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of COX4 in Lane A: Jurkat Whole Cell Lysate (30 µg). Samples were probed using a COX4 Polyclonal Antibody (Product # PA5-80580) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:24 kDa. Observed band size:24 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using COX4NB Polyclonal Antibody (Product # PA5-80580) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 24 kDa. (We are unsure of the identity of these extra bands).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human COX4NB in human pancreas with COX4NB Polyclonal Antibody (Product # PA5-80580, 1:2,000, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human COX4NB in human placenta with COX4NB Polyclonal Antibody (Product # PA5-80580, 1:2,000, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human COX4NB in human skeletal muscle with COX4NB Polyclonal Antibody (Product # PA5-80580, 1:2,000, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COX4NB Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 2 µL with COX4NB Polyclonal Antibody (Product # PA5-80580) and 15 µL of 50 % Protein G agarose. Primary antibody: COX4NB Polyclonal Antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 24 kDa.