PA5-97606

antibody from Invitrogen Antibodies

Targeting: EMC8 C16orf2, C16orf4, COX4NB, FAM158B, NOC4

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-97606

Provider

Invitrogen Antibodies

Product name

COX4NB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

4 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of COX4 using a COX4 Polyclonal antibody (Product # PA5-97606) at a concentration of 4 µg/mL. Lane 1: A549 whole cell lysate. Lane 2: Human placenta tissue. Lane 3: Hela whole cell lysate. Lane 4: HT29 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:10,000 dilution. Observed band size: 24 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of COX4 using a COX4 Polyclonal antibody (Product # PA5-97606) at a concentration of 4 µg/mL. Lane 1: A549 whole cell lysate. Lane 2: Human placenta tissue. Lane 3: Hela whole cell lysate. Lane 4: HT29 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:10,000 dilution. Observed band size: 24 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of COX4 in MCF-7 cells using a COX4 polyclonal antibody (Product # PA5-97606) at a dilution of 1:133. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of COX4 in paraffin embedded human adrenal gland tissue using a COX4 polyclonal antibody (Product # PA5-97606) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of COX4 in paraffin embedded human cervical cancer using a COX4 polyclonal antibody (Product # PA5-97606) at a dilution of 1:100.