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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- PA5-23477 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TMEM26 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Tmem26 in Lysate from human brain in the 1) absence and 2) presence of immunizing peptide using a Tmem26 polyclonal antibody (Product # PA5-23477) at 2 µg/mL. Goat anti-rabbit Ig HRP secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TMEM26 in human Testis, Spleen, Colon, Kidney and Brain. Samples were incubated in TMEM26 polyclonal antibody (Product # PA5-23477) using a dilution of 2 µg/mL followed by an anti-rabbit HRP secondary antibody. Separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. Detection: chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TMEM26 in 0.5 mg/mL Human Brain lysate. Samples were incubated in TMEM26 polyclonal antibody (Product # PA5-23477). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
