Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 681102 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#681102, RRID:AB_2566443
- Product name
- Purified anti-FGF-19
- Antibody type
- Monoclonal
- Antigen
- Partial human FGF-19 recombinant protein (25-216 a.a.) expressed in E. coli.
- Description
- FGF-19 belongs to a subfamily of the fibroblast growth factors (FGFs) called endocrine FGFs, which consists of FGF-19, FGF-21, and FGF-23. Unlike other subfamily of FGFs, FGF-19 has a low binding affinity to heparin sulfate and functions as a circulating hormone with effects on distant tissues. FGF-19 has reduced binding affinity to the FGF receptors and the interaction requires two necessary cofactors that are α-Klotho and β-Klotho. In the ileum, FGF-19 expression is initiated by bile acids-induced activation of farnesoid X receptor (FXR). In response to bile acid stimulation, FGF-19 is secreted and enters the portal venous circulation, which subsequently down-regulates hepatic bile acid synthesis through repression of the CYP7A1 gene encoding cholesterol 7-alpha-monooxygenase. The impaired regulation of bile acids by FGF-19 leads to bile acid diarrhea also known as idiopathic bile acid malabsorption. FGF-19 has also been demonstrated to regulate carbohydrate and lipid metabolism.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 13D9C80
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- 20 or 50 ng of human FGF-19 recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-human FGF-19 (clone 13D9C80) antibody. Proteins were visualized using a HRP conjugated anti-mouse-IgG secondary and chemiluminescence detection.
- Conjugate
- Unconjugated