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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- ARG55799 - Provider product page
- Provider
- Arigo
- Proper citation
- Arigo Cat#ARG55799, RRID:AB_2756411
- Product name
- anti-LC3B antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide of Human LC3B (NP_073729.1)
- Description
- Affinity purification with immunogen.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 30 µg of 1) SW620, 2) Mouse spleen, and 3) Rat spleen lysates stained with ARG55799 anti-LC3B antibody at 1:1000 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 30 µg of HeLa untreated or treated with Chloroquine at 50 µM, 100 µM (18 hr). The bolts stained with ARG55799 anti-LC3B antibody at 1:1000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: U20S cell stained with ARG55799 anti-LC3B antibody. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Rat kidney stained with ARG55799 anti-LC3B antibody at 1:100 dilution.
