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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- IC9390R-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human LC3B Alexa Fluor® 647-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human LC3B in flow cytometry.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Red dye
- Antigen sequence
Q9GZQ8- Isotype
- IgG
- Antibody clone number
- 1251A
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of LC3B in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line either treated with 50 μM chloroquine for 24 hours (filled histogram) or untreated (open histogram) was stained with Rabbit Anti-Human LC3B Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC9390R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
