Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PAB12534 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB12534, RRID:AB_10551913
- Product name
- MAP1LC3B polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MAP1LC3B.
- Storage
- Store at -20°C or -80°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Induction of autophagic cell death by a novel molecule is increased by hypoxia.
OSU-03012, a novel celecoxib derivative, induces reactive oxygen species-related autophagy in hepatocellular carcinoma.
Compensatory activation of ERK1/2 in Atg5-deficient mouse embryo fibroblasts suppresses oxidative stress-induced cell death.
Evidence that curcumin suppresses the growth of malignant gliomas in vitro and in vivo through induction of autophagy: role of Akt and extracellular signal-regulated kinase signaling pathways.
Tafani M, Schito L, Anwar T, Indelicato M, Sale P, Di Vito M, Morgante E, Beraldi R, Makovec F, Letari O, Caselli G, Spadafora C, Pucci B, Russo MA
Autophagy 2008 Nov;4(8):1042-53
Autophagy 2008 Nov;4(8):1042-53
OSU-03012, a novel celecoxib derivative, induces reactive oxygen species-related autophagy in hepatocellular carcinoma.
Gao M, Yeh PY, Lu YS, Hsu CH, Chen KF, Lee WC, Feng WC, Chen CS, Kuo ML, Cheng AL
Cancer research 2008 Nov 15;68(22):9348-57
Cancer research 2008 Nov 15;68(22):9348-57
Compensatory activation of ERK1/2 in Atg5-deficient mouse embryo fibroblasts suppresses oxidative stress-induced cell death.
Pyo JO, Nah J, Kim HJ, Lee HJ, Heo J, Lee H, Jung YK
Autophagy 2008 Apr;4(3):315-21
Autophagy 2008 Apr;4(3):315-21
Evidence that curcumin suppresses the growth of malignant gliomas in vitro and in vivo through induction of autophagy: role of Akt and extracellular signal-regulated kinase signaling pathways.
Aoki H, Takada Y, Kondo S, Sawaya R, Aggarwal BB, Kondo Y
Molecular pharmacology 2007 Jul;72(1):29-39
Molecular pharmacology 2007 Jul;72(1):29-39
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MAP1LC3B in treated U87 MG (human glioblastoma astrocytoma) lysates using MAP1LC3B polyclonal antibody (Cat # PAB12534).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MAP1LC3B on treated U373-MG cells using MAP1LC3B polyclonal antibody (Cat # PAB12534). The nuclei were stained with DAPI.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- LC3B staining of MAP1LC3B in gliobastoma multiform tissue using MAP1LC3B polyclonal antibody (Cat # PAB12534).
- Validation comment
- Immunohistochemistry
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Staining of MAP1LC3B on treated U373-MG (human glioblastoma) cells using MAP1LC3B polyclonal antibody (Cat # PAB12534).
- Validation comment
- Immunohistochemistry
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- LC3B staining of MAP1LC3B in treated U87-MG cultured & subcutaneous tumors using MAP1LC3B polyclonal antibody (Cat # PAB12534).
- Validation comment
- Immunohistochemistry