MAB85582-100

antibody from R&D Systems

Targeting: MAP1LC3B ATG8F

Antibody data

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Validation data

Product number: MAB85582-100 - Provider product page
Provider: R&D Systems
Product name: Human/Mouse/Rat LC3B Antibody
Antibody type: Monoclonal
Description: Protein A or G purified from cell culture supernatant. Detects human, mouse, and rat LC3B in Western blots.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: Q9GZQ8
Isotype: IgG
Antibody clone number: 1251B
Vial size: 100 ug
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

MAP1LC3B protein structure - MAB85582-100 shown in red.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of Mouse and Rat LC3B by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat LC3B Monoclonal Antibody (Catalog # MAB85582) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of Mouse LC3B by Simple Western^TM. Simple Western lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 hours, loaded at 0.2 mg/mL. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat LC3B Monoclonal Antibody (Catalog # MAB85582) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of Human LC3B by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 Hours. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat LC3B Monoclonal Antibody (Catalog # MAB85582) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Western Blow Show Human LC3B Specificty Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat LC3B Monoclonal Antibody (Catalog # MAB85582) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.