Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [8]
- Immunocytochemistry [4]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- 18725-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LC3B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.3 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chloroquine treated HeLa cells were subjected to SDS PAGE followed by western blot with 18725-1-AP ( MAP1LC3B-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TN treated Hela cells were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 18725-1-AP (LC3B-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using 18725-1-AP (LC3B-Specific antibody; green) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). Cells was co-stained with alpha-tubulin (66031-1-Ig; red).
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of starvation treated HEK-293 cells using 18725-1-AP (LC3B-Specific Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). Cells were fixed with ethanol at -20 °C. 20 mM chloroquine was used to block the autophagy flux.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed Starvation treated HepG2 cells using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed Starvation treated HepG2 cells using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 18725-1-AP (LC3B-Specific antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were intracellularly stained with 0.4 µg Anti-Human LC3B-Specific (Product # 18725-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (red), or 0.4 µg Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).