GTX127375

antibody from GeneTex

Targeting: MAP1LC3B ATG8F

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX127375

Provider

GeneTex

Proper citation

GeneTex Cat#GTX127375, RRID:AB_11176277

Product name

LC3B antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat, Porcine

Host

Rabbit

References

Submitted references

Inhibition of autophagy protects against sepsis by concurrently attenuating the cytokine storm and vascular leakage.

Lu LH, Chao CH, Yeh TM
The Journal of infection 2019 Mar;78(3):178-186

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Chloramphenicol Induces Autophagy and Inhibits the Hypoxia Inducible Factor-1 Alpha Pathway in Non-Small Cell Lung Cancer Cells.

Hsu HL, Liao PL, Cheng YW, Huang SH, Wu CH, Li CH, Kang JJ
International journal of molecular sciences 2019 Jan 3;20(1)

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Recycling endosomal CD133 functions as an inhibitor of autophagy at the pericentrosomal region.

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Scientific reports 2019 Feb 19;9(1):2236

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Autophagy regulates vinorelbine sensitivity due to continued Keap1-mediated ROS generation in lung adenocarcinoma cells.

Wu YW, Lin CF, Lin YS, Su WC, Chiu WH
Cell death discovery 2018;4:33

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Sodium Iodate Disrupted the Mitochondrial-Lysosomal Axis in Cultured Retinal Pigment Epithelial Cells.

Lin YC, Horng LY, Sung HC, Wu RT
Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics 2018 Sep;34(7):500-511

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Role of Dicer in regulating oxaliplatin resistance of colon cancer cells.

Lai HH, Lin LJ, Hung LY, Chen PS
Biochemical and biophysical research communications 2018 Nov 17;506(1):87-93

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Dual Inhibition of PIK3C3 and FGFR as a New Therapeutic Approach to Treat Bladder Cancer.

Chen CH, Changou CA, Hsieh TH, Lee YC, Chu CY, Hsu KC, Wang HC, Lin YC, Lo YN, Liu YR, Liou JP, Yen Y
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Mar 1;24(5):1176-1189

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Modulation of Macrophage Phenotype by Biodegradable Polyurethane Nanoparticles: Possible Relation between Macrophage Polarization and Immune Response of Nanoparticles.

Huang YJ, Hung KC, Hung HS, Hsu SH
ACS applied materials & interfaces 2018 Jun 13;10(23):19436-19448

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Minocycline suppresses dengue virus replication by down-regulation of macrophage migration inhibitory factor-induced autophagy.

Lai YC, Chuang YC, Chang CP, Lin YS, Perng GC, Wu HC, Hsieh SL, Yeh TM
Antiviral research 2018 Jul;155:28-38

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Liposomal delivery of a Pin1 inhibitor complexed with cyclodextrins as new therapy for high-grade serous ovarian cancer.

Russo Spena C, De Stefano L, Palazzolo S, Salis B, Granchi C, Minutolo F, Tuccinardi T, Fratamico R, Crotti S, D'Aronco S, Agostini M, Corona G, Caligiuri I, Canzonieri V, Rizzolio F
Journal of controlled release : official journal of the Controlled Release Society 2018 Jul 10;281:1-10

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Lycium barbarum polysaccharide protects against oxygen glucose deprivation/reoxygenation-induced apoptosis and autophagic cell death via the PI3K/Akt/mTOR signaling pathway in primary cultured hippocampal neurons.

Yu Y, Wu X, Pu J, Luo P, Ma W, Wang J, Wei J, Wang Y, Fei Z
Biochemical and biophysical research communications 2018 Jan 1;495(1):1187-1194

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A mitohormetic response to pro-oxidant exposure in the house mouse.

Zhang Y, Humes F, Almond G, Kavazis AN, Hood WR
American journal of physiology. Regulatory, integrative and comparative physiology 2018 Jan 1;314(1):R122-R134

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Extracellular acidification by lactic acid suppresses glucose deprivation-induced cell death and autophagy in B16 melanoma cells.

Matsuo T, Sadzuka Y
Biochemical and biophysical research communications 2018 Feb 19;496(4):1357-1361

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miR-30a Remodels Subcutaneous Adipose Tissue Inflammation to Improve Insulin Sensitivity in Obesity.

Koh EH, Chernis N, Saha PK, Xiao L, Bader DA, Zhu B, Rajapakshe K, Hamilton MP, Liu X, Perera D, Chen X, York B, Trauner M, Coarfa C, Bajaj M, Moore DD, Deng T, McGuire SE, Hartig SM
Diabetes 2018 Dec;67(12):2541-2553

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Autophagy mediates cell cycle response by regulating nucleocytoplasmic transport of PAX6 in limbal stem cells under ultraviolet-A stress.

Laggner M, Pollreisz A, Schmidinger G, Schmidt-Erfurth U, Chen YT
PloS one 2017;12(7):e0180868

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6-shogaol induces autophagic cell death then triggered apoptosis in colorectal adenocarcinoma HT-29 cells.

Li TY, Chiang BH
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2017 Sep;93:208-217

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Endoplasmic reticulum-targeting photosensitizer Hypericin confers chemo-sensitization towards oxaliplatin through inducing pro-death autophagy.

Lin S, Yang L, Shi H, Du W, Qi Y, Qiu C, Liang X, Shi W, Liu J
The international journal of biochemistry & cell biology 2017 Jun;87:54-68

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The inhibition of lung cancer cell migration by AhR-regulated autophagy.

Tsai CH, Li CH, Cheng YW, Lee CC, Liao PL, Lin CH, Huang SH, Kang JJ
Scientific reports 2017 Feb 14;7:41927

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Epigallocatechin-3-Gallate Suppresses Human Herpesvirus 8 Replication and Induces ROS Leading to Apoptosis and Autophagy in Primary Effusion Lymphoma Cells.

Tsai CY, Chen CY, Chiou YH, Shyu HW, Lin KH, Chou MC, Huang MH, Wang YF
International journal of molecular sciences 2017 Dec 21;19(1)

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Fisetin Protects PC12 Cells from Tunicamycin-Mediated Cell Death via Reactive Oxygen Species Scavenging and Modulation of Nrf2-Driven Gene Expression, SIRT1 and MAPK Signaling in PC12 Cells.

Yen JH, Wu PS, Chen SF, Wu MJ
International journal of molecular sciences 2017 Apr 17;18(4)

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EZH2 inhibitors transcriptionally upregulate cytotoxic autophagy and cytoprotective unfolded protein response in human colorectal cancer cells.

Hsieh YY, Lo HL, Yang PM
American journal of cancer research 2016;6(8):1661-80

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The Inhibition of microRNA-128 on IGF-1-Activating mTOR Signaling Involves in Temozolomide-Induced Glioma Cell Apoptotic Death.

Chen PH, Cheng CH, Shih CM, Ho KH, Lin CW, Lee CC, Liu AJ, Chang CK, Chen KC
PloS one 2016;11(11):e0167096

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Mycotoxin Patulin Suppresses Innate Immune Responses by Mitochondrial Dysfunction and p62/Sequestosome-1-dependent Mitophagy.

Tsai WT, Lo YC, Wu MS, Li CY, Kuo YP, Lai YH, Tsai Y, Chen KC, Chuang TH, Yao CH, Lee JC, Hsu LC, Hsu JT, Yu GY
The Journal of biological chemistry 2016 Sep 9;291(37):19299-311

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Dual Roles of 17-β Estradiol in Estrogen Receptor-dependent Growth Inhibition in Renal Cell Carcinoma.

Chen KC, Lin CM, Huang CJ, Chen SK, Wu ST, Chiang HS, Ku WC
Cancer genomics & proteomics 2016 May-Jun;13(3):219-30

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The ALS/FTLD associated protein C9orf72 associates with SMCR8 and WDR41 to regulate the autophagy-lysosome pathway.

Sullivan PM, Zhou X, Robins AM, Paushter DH, Kim D, Smolka MB, Hu F
Acta neuropathologica communications 2016 May 18;4(1):51

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Systematic discovery of drug action mechanisms by an integrated chemical genomics approach: identification of functional disparities between azacytidine and decitabine.

Hsieh YY, Huang TC, Lo HL, Jhan JY, Chen ST, Yang PM
Oncotarget 2016 May 10;7(19):27363-78

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Dengue Virus Nonstructural Protein 1 Induces Vascular Leakage through Macrophage Migration Inhibitory Factor and Autophagy.

Chen HR, Chuang YC, Lin YS, Liu HS, Liu CC, Perng GC, Yeh TM
PLoS neglected tropical diseases 2016 Jul;10(7):e0004828

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Progress of endocytic CHRN to autophagic degradation is regulated by RAB5-GTPase and T145 phosphorylation of SH3GLB1 at mouse neuromuscular junctions in vivo.

Wild F, Khan MM, Straka T, Rudolf R
Autophagy 2016 Dec;12(12):2300-2310

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Enhanced Autophagy of Adipose-Derived Stem Cells Grown on Chitosan Substrates.

Yang CM, Huang YJ, Hsu SH
BioResearch open access 2015;4(1):89-96

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Luteolin and Apigenin Attenuate 4-Hydroxy-2-Nonenal-Mediated Cell Death through Modulation of UPR, Nrf2-ARE and MAPK Pathways in PC12 Cells.

Wu PS, Yen JH, Kou MC, Wu MJ
PloS one 2015;10(6):e0130599

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LC3 overexpression reduces Aβ neurotoxicity through increasing α7nAchR expression and autophagic activity in neurons and mice.

Hung SY, Huang WP, Liou HC, Fu WM
Neuropharmacology 2015 Jun;93:243-51

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S-Adenosyl-L-methionine-competitive inhibitors of the histone methyltransferase EZH2 induce autophagy and enhance drug sensitivity in cancer cells.

Liu TP, Lo HL, Wei LS, Hsiao HH, Yang PM
Anti-cancer drugs 2015 Feb;26(2):139-47

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Carboxyl-functionalized polyurethane nanoparticles with immunosuppressive properties as a new type of anti-inflammatory platform.

Huang YJ, Hung KC, Hsieh FY, Hsu SH
Nanoscale 2015 Dec 28;7(48):20352-64

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A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine.

Lee KH, Lo HL, Tang WC, Hsiao HH, Yang PM
Scientific reports 2014 Sep 17;4:6394

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Spheroid formation and enhanced cardiomyogenic potential of adipose-derived stem cells grown on chitosan.

Liu BH, Yeh HY, Lin YC, Wang MH, Chen DC, Lee BH, Hsu SH
BioResearch open access 2013 Feb;2(1):28-39

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Eburicoic Acid, an Active Triterpenoid from the Fruiting Bodies of Basswood Cultivated Antrodia cinnamomea, Induces ER Stress-Mediated Autophagy in Human Hepatoma Cells.

Su YC, Liu CT, Chu YL, Raghu R, Kuo YH, Sheen LY
Journal of traditional and complementary medicine 2012 Oct;2(4):312-22

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

LC3B antibody detects LC3B protein in HCV-infected samples by western blot analysis. ? A. 20 ?g Huh7 whole cell lysate/extract (un-infected) ? B. 20 ?g Huh7 whole cell lysate/extract (HCV-infected) ? LC3B antibody (GTX127375) dilution: 1:1500 ?The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

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GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 15% SDS PAGE GTX127375 diluted at 1:1000

Validation comment

WB

Supportive validation

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Main image

Experimental details

Sample (30 ug of whole cell lysate) A: HeLa B: HeLa cells with tumicamycin trearment 8hr 15% SDS PAGE GTX127375 diluted at 1:5000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

LC3B antibody detects MAP1LC3B protein by western blot analysis.A. 20 ?g Huh7 whole cell lysate/extract (untreated) B. 20 ?g Huh7 whole cell lysate/extract (3uM-Thapsigargin treatment for 12hr)LC3B antibody (GTX127375) dilution: 1:1500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

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GeneTex (provider)

Main image

Experimental details

LC3B antibody detects MAP1LC3B protein by western blot analysis.A. 50 ?g mouse brain lysate/extract15% SDS-PAGELC3B antibody (GTX127375) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

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GeneTex (provider)

Main image

Experimental details

LC3B antibody detects MAP1LC3B protein by western blot analysis.A. 50 ?g Rat brain lysate/extract15% SDS-PAGELC3B antibody (GTX127375) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

LC3B antibody detects LC3B protein in Thapsigargin-treated samples by western blot analysis.Upper panel: LC3B antibody (GTX127375)Lower panel: Beta-actin antibody (GTX110564)A. 30 ?g HepG2 whole cell lysate/extract (untreated) B. 30 ?g HepG2 whole cell lysate/extract (3 ?M Thapsigargin treatment for 12 hr)15% SDS-PAGELC3B antibody (GTX127375) dilution: 1:1000Beta-actin antibody (GTX110564) dilution: 1:20000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

LC3B antibody detects LC3B protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

LC3B antibody detects LC3B protein by western blot analysis. Un-treated (-) and treated (+, 50uM Chloroquine treatment for 48 hr) HeLa whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) at a dilution of 1:500.The beta Actin was used as internal control (GTX10564, 1:50000) shown at the bottom panel.

Validation comment

WB

Supportive validation

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GeneTex (provider)

Main image

Experimental details

HepG2 cells were untreated or treated with 3 £gM thapsigargin for 16 and 24 hrs. Whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Untreated (¡V) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody (GTX127375) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

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Main image

Experimental details

LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: Hep G2 cells mock (left) and treated with 3 £gM Thapsigargin for 12 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with ice-cold acetone for 1 min.Green: LC3B protein stained by LC3B antibody (GTX127375) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Supportive validation

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Main image

Experimental details

LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50£gM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: LC3B protein stained by LC3B antibody (GTX127375) diluted at 1:2000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.

Supportive validation

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Main image

Experimental details

LC3B antibody detects LC3B protein at autophagosome by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: LC3B protein stained by LC3B antibody (GTX127375) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, diluted at 1:50.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Immunoprecipitation

Supportive validation

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Experimental details

Immunoprecipitation of LC3B protein from U87-MG whole cell extracts using 5 £gg of LC3B antibody (GTX127375).Western blot analysis was performed using LC3B antibody (GTX127375).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemistry

Supportive validation

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Experimental details

LC3B antibody detects LC3B protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. LC3B antibody (GTX127375) diluted at 1:500.

Flow cytometry

Supportive validation

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Main image

Experimental details

LC3B antibody (GTX127375) detects LC3B protein by flow cytometry analysis. Sample: HeLa cell fixed in 4% paraformaldehyde at 4?C for 5 min. Brown: Unlabelled sample was also used as a control. Blue: LC3B antibody (GTX127375) dilution: 1:100. Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.