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- References [7]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- GTX82986 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX82986, RRID:AB_848127
- Product name
- LC3B antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Porcine, Simian, Zebrafish
- Host
- Rabbit
Submitted references Tyrosinase-Cre-Mediated Deletion of the Autophagy Gene Atg7 Leads to Accumulation of the RPE65 Variant M450 in the Retinal Pigment Epithelium of C57BL/6 Mice.
Autophagy facilitates secretion and protects against degeneration of the Harderian gland.
Autophagy is induced by UVA and promotes removal of oxidized phospholipids and protein aggregates in epidermal keratinocytes.
Epidermal keratinocytes form a functional skin barrier in the absence of Atg7 dependent autophagy.
Autophagy mediates survival of pancreatic tumour-initiating cells in a hypoxic microenvironment.
Blockade of fatty acid synthase induces ubiquitination and degradation of phosphoinositide-3-kinase signaling proteins in ovarian cancer.
GSK-3beta promotes cell survival by modulating Bif-1-dependent autophagy and cell death.
Sukseree S, Chen YT, Laggner M, Gruber F, Petit V, Nagelreiter IM, Mlitz V, Rossiter H, Pollreisz A, Schmidt-Erfurth U, Larue L, Tschachler E, Eckhart L
PloS one 2016;11(8):e0161640
PloS one 2016;11(8):e0161640
Autophagy facilitates secretion and protects against degeneration of the Harderian gland.
Koenig U, Fobker M, Lengauer B, Brandstetter M, Resch GP, Gröger M, Plenz G, Pammer J, Barresi C, Hartmann C, Rossiter H
Autophagy 2015;11(2):298-313
Autophagy 2015;11(2):298-313
Autophagy is induced by UVA and promotes removal of oxidized phospholipids and protein aggregates in epidermal keratinocytes.
Zhao Y, Zhang CF, Rossiter H, Eckhart L, König U, Karner S, Mildner M, Bochkov VN, Tschachler E, Gruber F
The Journal of investigative dermatology 2013 Jun;133(6):1629-37
The Journal of investigative dermatology 2013 Jun;133(6):1629-37
Epidermal keratinocytes form a functional skin barrier in the absence of Atg7 dependent autophagy.
Rossiter H, König U, Barresi C, Buchberger M, Ghannadan M, Zhang CF, Mlitz V, Gmeiner R, Sukseree S, Födinger D, Eckhart L, Tschachler E
Journal of dermatological science 2013 Jul;71(1):67-75
Journal of dermatological science 2013 Jul;71(1):67-75
Autophagy mediates survival of pancreatic tumour-initiating cells in a hypoxic microenvironment.
Rausch V, Liu L, Apel A, Rettig T, Gladkich J, Labsch S, Kallifatidis G, Kaczorowski A, Groth A, Gross W, Gebhard MM, Schemmer P, Werner J, Salnikov AV, Zentgraf H, Büchler MW, Herr I
The Journal of pathology 2012 Jul;227(3):325-35
The Journal of pathology 2012 Jul;227(3):325-35
Blockade of fatty acid synthase induces ubiquitination and degradation of phosphoinositide-3-kinase signaling proteins in ovarian cancer.
Tomek K, Wagner R, Varga F, Singer CF, Karlic H, Grunt TW
Molecular cancer research : MCR 2011 Dec;9(12):1767-79
Molecular cancer research : MCR 2011 Dec;9(12):1767-79
GSK-3beta promotes cell survival by modulating Bif-1-dependent autophagy and cell death.
Yang J, Takahashi Y, Cheng E, Liu J, Terranova PF, Zhao B, Thrasher JB, Wang HG, Li B
Journal of cell science 2010 Mar 15;123(Pt 6):861-70
Journal of cell science 2010 Mar 15;123(Pt 6):861-70
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Western Blot detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates
Supportive validation
- Submitted by
- GeneTex (provider)
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- Experimental details
- ICC/IF staining of untreated or C2-Ceramide-treated U373-MG cells using LC3B antibody (GTX82986). Nuclei stained with DAPI (Blue).
Supportive validation
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- GeneTex (provider)
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- Immunohistochemistry: LC3B Antibody [GTX82986] - LC3B staining in treated U87-MG cultured & subcutaneous tumors.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry: LC3B Antibody [GTX82986] - LC3B staining in gliobastoma multiform tissue.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry: LC3B Antibody [GTX82986] - Staining of treated U373-MG (human glioblastoma.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow Cytometry: LC3B Antibody [GTX82986] - Staining of NTERA-2 cells using GTX82986 at a 1:200 dilution detected using Dylight-488 conjμgated goat anti-rabbit IgG secondary.
