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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-35032 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP2B6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references In vitro toxicological evaluation of NCS-382, a high-affinity antagonist of γ-hydroxybutyrate (GHB) binding.
Vogel KR, Ainslie GR, Roullet JB, McConnell A, Gibson KM
Toxicology in vitro : an international journal published in association with BIBRA 2017 Apr;40:196-202
Toxicology in vitro : an international journal published in association with BIBRA 2017 Apr;40:196-202
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cytochrome P450 2B6 in 293 (lane 1) and K562 (lane 2) cell lysates (35 µg/lane) using a Cytochrome P450 2B6 polyclonal antibody (Product # PA5-35032).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts of Hep G2 (Lane 1), MCF7 (Lane 2), COLO 205 (Lane 3), K562 (Lane 4), HEK 293 (Lane 5), Jurkat (Lane 6), tissue extracts of Mouse Liver (Lane 7) and Rat Liver (Lane 8). The blot was probed with Anti-CYP2B6 Polyclonal Antibody (Product # PA5-35032, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 70 kDa band corresponding to CYP2B6 was detected across the cell lines and tissues tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cytochrome P450 2B6 in 293 cells using a Cytochrome P450 2B6 polyclonal antibody (Product # PA5-35032) followed by detection using a fluorescent conjugated secondary antibody (green). Nuclei were stained with Dapi (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Cytochrome P450 2B6 in formalin-fixed, paraffin-embedded human hepatocarcinoma using a Cytochrome P450 2B6 polyclonal antibody (Product # PA5-35032) followed by peroxidase conjugation of the secondary antibody and DAB staining.
