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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00054758-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00054758-M02, RRID:AB_626470
- Product name
- KLHDC4 monoclonal antibody (M02), clone 4G11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant KLHDC4.
- Antigen sequence
MGKKGKKEKKGRGAEKTAAKMEKKVSKRSRKEETF
LYNELYVYNTRKDTWTKVDIPSPPPRRCAHQAVVV
PQGGGQLWVFGGEFASPNGEQFYHYKDLWVLHLAT
KTWEQVKSTGGPSGRSGHRMVAWKRQLILFGGFHE
STRDYIYYNDVYAFNLDTFTWSKLSPSGTGPTPRS
GCQMSVTPQGGIVVYGGYSKQRVKKDVDKGTRHSD
MFLLKPEDGREDKWVWTRMNPSGVKPTPRSGFSVA
MAPNHQTLFFGGVCDEEEEESLSGEFFNDLYFYDA
TRNRWFEGQLKGPKSEKKKRRRGRKEEPEGGSRPA
CGGAGTQGPVQLVKEVVAEDGTVVTIKQVLTAPGS
AGQPRSEDEDSLEEAGSPAPGPCPRSNAMLAVKHG
VLYVYGGMFEAGDRQVTLSDLHCLDLHRMEAWKAL
VEMDPETQEWLEETDSEEDSEEVEGAEGGVDDEDS
GEESGAED- Isotype
- IgG
- Antibody clone number
- 4G11
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of KLHDC4 expression in transfected 293T cell line by KLHDC4 monoclonal antibody (M02), clone 4G11.Lane 1: KLHDC4 transfected lysate(57.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged KLHDC4 is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to KLHDC4 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to KLHDC4 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.5 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
