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LearnA300-279A
antibody from Invitrogen Antibodies
Targeting: BANP
BEND1, DKFZp761H172, FLJ10177, FLJ20538, SMAR1, SMARBP1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- A300-279A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMAR1/BANP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human, mouse and rat SMAR1/BANP by western blot. Samples: Whole cell lysate (50 µg) from mouse 4T1, mouse CT26.WT, rat C6, HeLa, and 293T cells. Antibodies: Affinity purified rabbit anti-SMAR1/BANP antibody A300-279A (lot A300-279A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SMAR1 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, Jurkat, MDA-MB-231, RKO, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMAR1 antibody (Product # A300-279A lot 4) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse SMAR1 by western blot. Samples: Whole cell lysate (25 µg) from NIH 3T3, TCMK-1, CT26, CH27, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMAR1 antibody (Product # A300-279A lot 4) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SMAR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-SMAR1 antibody A300-279A (lot A300-279A-2) used for IP at 6 µg/mg lysate. SMAR1 was also immunoprecipitated by a previous lot (lot A300-279A-1). For blotting immunoprecipitated SMAR1, A300-279A-2 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human, mouse and rat SMAR1/BANP by western blot. Samples: Whole cell lysate (50 µg) from mouse 4T1, mouse CT26.WT, rat C6, HeLa, and 293T cells. Antibodies: Affinity purified rabbit anti-SMAR1/BANP antibody A300-279A (lot A300-279A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SMAR1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibodies: Affinity purified rabbit anti-SMAR1 (A300-279A; lot 4) used at dilution of 1:5,000 (0.2 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse SMAR1 by immunohistochemistry. Sample: FFPE sections of human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-SMAR1 (Cat. No. A300-279A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SMAR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMAR1 antibody (Produce # A300-279A lot 4) used for IP at 6 µg per reaction. SMAR1 was also immunoprecipitated by a previous lot of this antibody (Product # A300-279A lot 3). For blotting immunoprecipitated SMAR1 (Product # A300-279A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
