Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 14917-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MEPCE Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: SGSLSAPPA ASVISAPPSS SSRHRKRRRT SSKSEAGARG GGQGSKEKGR GSWGGRHHHH HPLPAAGFKK QQRKFQYGNY CKYYGYRNPS CEDGRLRVLK PEWFRGRDVL DLGCNVGHLT LSIACKWGPS RMVGLDIDSR LIHSARQNIR HYLSEELRLP PQTLEGDPGA EGEEGTTTVR KRSCFPASLT ASRGPIAAPQ VPLDGADTSV FPNNVVFVTG NYVLDRDDLV EAQTPEYDVV LCLSLTKWVH LNWGDEGLKR MFRRIYRHLR PGGILVLEPQ PWSSYGKRKT LTETIYKNYY RIQLKPEQFS SYLTSPDVGF SSYELVATPH NTSKGFQRPV YLFHKARSPS H (340-689 aa encoded by BC000556)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.67 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 14917-1-AP (MEPCE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 14917-1-AP (MEPCE antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed MCF-7 cells using 14917-1-AP (MEPCE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-MEPCE (IP:14917-1-AP, 4ug; Detection:14917-1-AP 1:400) with MCF-7 cells lysate 3200ug.