Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- GTX129407 - Provider product page
- Provider
- GeneTex
- Product name
- AXL antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Ampelopsins A and C Induce Apoptosis and Metastasis through Downregulating AxL, TYRO3, and FYN Expressions in MDA-MB-231 Breast Cancer Cells.
Huang C, Huang YL, Wang CC, Pan YL, Lai YH, Huang HC
Journal of agricultural and food chemistry 2019 Mar 13;67(10):2818-2830
Journal of agricultural and food chemistry 2019 Mar 13;67(10):2818-2830
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- AXL antibody detects AXL protein by western blot analysis.A. 30 £gg 293T whole cell extractB. 30 £gg whole cell extract of human AXL-transfected 293T cells7.5 % SDS-PAGEAXL antibody (GTX129407) dilution: 1:5000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- AXL antibody detects AXL protein by western blot analysis.A. 30 £gg A549 whole cell extractB. 30 £gg H1299 whole cell extractC. 30 £gg HCT116 whole cell extract5 % SDS-PAGEAXL antibody (GTX129407) dilution: 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 5% SDS-PAGE, and the membrane was blotted with AXL antibody (GTX129407) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- An ELISA plate is coated with 50 £gL of AXL recombinant protein at concentration of 4 £gg/mL. The coated protein is detected with anti-AXL antibody (GTX129407) at concentration ranged from 0.25 to 1000 ng/mL.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- AXL antibody detects AXL protein at membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. AXL antibody (GTX129407) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- AXL antibody detects AXL protein at cell membrane on human non-small cell lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human non-small cell lung carcinoma. AXL antibody (GTX129407) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- AXL antibody detects AXL protein at membrane on human non-small cell lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human non-small cell lung carcinoma. AXL antibody (GTX129407) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- AXL antibody detects AXL protein at cell membrane in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Green: AXL antibody (GTX129407) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).Blue: Nuclear staining with Hoechst 33342.