AF154

antibody from R&D Systems

Targeting: AXL ARK, JTK11, Tyro7, UFO

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

AF154

Provider

R&D Systems

Product name

Human Axl Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Axl in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse Axl is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

AAA61243


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

p5RHH nanoparticle-mediated delivery of AXL siRNA inhibits metastasis of ovarian and uterine cancer cells in mouse xenografts.

Mills KA, Quinn JM, Roach ST, Palisoul M, Nguyen M, Noia H, Guo L, Fazal J, Mutch DG, Wickline SA, Pan H, Fuh KC
Scientific reports 2019 Mar 18;9(1):4762

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Inhibition of MERTK Promotes Suppression of Tumor Growth in BRAF Mutant and BRAF Wild-Type Melanoma.

Sinik L, Minson KA, Tentler JJ, Carrico J, Bagby SM, Robinson WA, Kami R, Burstyn-Cohen T, Eckhardt SG, Wang X, Frye SV, Earp HS, DeRyckere D, Graham DK
Molecular cancer therapeutics 2019 Feb;18(2):278-288

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Infection by Zika viruses requires the transmembrane protein AXL, endocytosis and low pH.

Persaud M, Martinez-Lopez A, Buffone C, Porcelli SA, Diaz-Griffero F
Virology 2018 May;518:301-312

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ATP-binding cassette member B5 (ABCB5) promotes tumor cell invasiveness in human colorectal cancer.

Guo Q, Grimmig T, Gonzalez G, Giobbie-Hurder A, Berg G, Carr N, Wilson BJ, Banerjee P, Ma J, Gold JS, Nandi B, Huang Q, Waaga-Gasser AM, Lian CG, Murphy GF, Frank MH, Gasser M, Frank NY
The Journal of biological chemistry 2018 Jul 13;293(28):11166-11178

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Protein S and Gas6 induce efferocytosis of HIV-1-infected cells.

Chua BA, Ngo JA, Situ K, Ramirez CM, Nakano H, Morizono K
Virology 2018 Feb;515:176-190

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Human Sertoli cells support high levels of Zika virus replication and persistence.

Kumar A, Jovel J, Lopez-Orozco J, Limonta D, Airo AM, Hou S, Stryapunina I, Fibke C, Moore RB, Hobman TC
Scientific reports 2018 Apr 3;8(1):5477

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Gas6 derived from cancer-associated fibroblasts promotes migration of Axl-expressing lung cancer cells during chemotherapy.

Kanzaki R, Naito H, Kise K, Takara K, Eino D, Minami M, Shintani Y, Funaki S, Kawamura T, Kimura T, Okumura M, Takakura N
Scientific reports 2017 Sep 6;7(1):10613

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A phase I study of foretinib plus erlotinib in patients with previously treated advanced non-small cell lung cancer: Canadian cancer trials group IND.196.

Leighl NB, Tsao MS, Liu G, Tu D, Ho C, Shepherd FA, Murray N, Goffin JR, Nicholas G, Sakashita S, Chen Z, Kim L, Powers J, Seymour L, Goss G, Bradbury PA
Oncotarget 2017 Sep 19;8(41):69651-69662

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Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation.

Cross SN, Potter JA, Aldo P, Kwon JY, Pitruzzello M, Tong M, Guller S, Rothlin CV, Mor G, Abrahams VM
Journal of immunology (Baltimore, Md. : 1950) 2017 Oct 15;199(8):2885-2895

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Inhibition of the GAS6/AXL pathway augments the efficacy of chemotherapies.

Kariolis MS, Miao YR, Diep A, Nash SE, Olcina MM, Jiang D, Jones DS 2nd, Kapur S, Mathews II, Koong AC, Rankin EB, Cochran JR, Giaccia AJ
The Journal of clinical investigation 2017 Jan 3;127(1):183-198

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Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication.

Aagaard KM, Lahon A, Suter MA, Arya RP, Seferovic MD, Vogt MB, Hu M, Stossi F, Mancini MA, Harris RA, Kahr M, Eppes C, Rac M, Belfort MA, Park CS, Lacorazza D, Rico-Hesse R
Scientific reports 2017 Jan 27;7:41389

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AXL-dependent infection of human fetal endothelial cells distinguishes Zika virus from other pathogenic flaviviruses.

Richard AS, Shim BS, Kwon YC, Zhang R, Otsuka Y, Schmitt K, Berri F, Diamond MS, Choe H
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 21;114(8):2024-2029

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Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma.

Dantas-Barbosa C, Lesluyes T, Loarer FL, Chibon F, Treilleux I, Coindre JM, Meeus P, Brahmi M, Bally O, Ray-Coquard I, Sunyach MP, Cesne AL, Mir O, Bonvalot S, Toulmonde M, Italiano A, Saintigny P, Jean-Denis M, Ducimetiere F, Ranchere D, El Sayadi H, Alberti L, Blay JY
British journal of cancer 2017 Dec 5;117(12):1787-1797

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TAM Receptors Are Not Required for Zika Virus Infection in Mice.

Hastings AK, Yockey LJ, Jagger BW, Hwang J, Uraki R, Gaitsch HF, Parnell LA, Cao B, Mysorekar IU, Rothlin CV, Fikrig E, Diamond MS, Iwasaki A
Cell reports 2017 Apr 18;19(3):558-568

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AXL receptor signalling suppresses p53 in melanoma through stabilization of the MDMX-MDM2 complex.

de Polo A, Luo Z, Gerarduzzi C, Chen X, Little JB, Yuan ZM
Journal of molecular cell biology 2017 Apr 1;9(2):154-165

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Clinical, Pathological, and Molecular Features of Lung Adenocarcinomas with AXL Expression.

Sato K, Suda K, Shimizu S, Sakai K, Mizuuchi H, Tomizawa K, Takemoto T, Nishio K, Mitsudomi T
PloS one 2016;11(4):e0154186

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ZEB1-mediated melanoma cell plasticity enhances resistance to MAPK inhibitors.

Richard G, Dalle S, Monet MA, Ligier M, Boespflug A, Pommier RM, de la Fouchardière A, Perier-Muzet M, Depaepe L, Barnault R, Tondeur G, Ansieau S, Thomas E, Bertolotto C, Ballotti R, Mourah S, Battistella M, Lebbé C, Thomas L, Puisieux A, Caramel J
EMBO molecular medicine 2016 Oct;8(10):1143-1161

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AXL modulates extracellular matrix protein expression and is essential for invasion and metastasis in endometrial cancer.

Divine LM, Nguyen MR, Meller E, Desai RA, Arif B, Rankin EB, Bligard KH, Meyerson C, Hagemann IS, Massad M, Thaker PH, Hagemann AR, McCourt CK, Powell MA, Mutch DG, Fuh KC
Oncotarget 2016 Nov 22;7(47):77291-77305

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The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles.

Happonen KE, Tran S, Mörgelin M, Prince R, Calzavarini S, Angelillo-Scherrer A, Dahlbäck B
The Journal of biological chemistry 2016 May 13;291(20):10586-601

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Cotargeting MNK and MEK kinases induces the regression of NF1-mutant cancers.

Lock R, Ingraham R, Maertens O, Miller AL, Weledji N, Legius E, Konicek BM, Yan SC, Graff JR, Cichowski K
The Journal of clinical investigation 2016 Jun 1;126(6):2181-90

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Implication of the Receptor Tyrosine Kinase AXL in Head and Neck Cancer Progression.

von Mässenhausen A, Brägelmann J, Billig H, Thewes B, Queisser A, Vogel W, Kristiansen G, Schröck A, Bootz F, Brossart P, Kirfel J, Perner S
International journal of molecular sciences 2016 Dec 22;18(1)

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Zika virus cell tropism in the developing human brain and inhibition by azithromycin.

Retallack H, Di Lullo E, Arias C, Knopp KA, Laurie MT, Sandoval-Espinosa C, Mancia Leon WR, Krencik R, Ullian EM, Spatazza J, Pollen AA, Mandel-Brehm C, Nowakowski TJ, Kriegstein AR, DeRisi JL
Proceedings of the National Academy of Sciences of the United States of America 2016 Dec 13;113(50):14408-14413

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The Axl-Regulating Tumor Suppressor miR-34a Is Increased in ccRCC but Does Not Correlate with Axl mRNA or Axl Protein Levels.

Fritz HK, Gustafsson A, Ljungberg B, Ceder Y, Axelson H, Dahlbäck B
PloS one 2015;10(8):e0135991

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MET and AXL inhibitor NPS-1034 exerts efficacy against lung cancer cells resistant to EGFR kinase inhibitors because of MET or AXL activation.

Rho JK, Choi YJ, Kim SY, Kim TW, Choi EK, Yoon SJ, Park BM, Park E, Bae JH, Choi CM, Lee JC
Cancer research 2014 Jan 1;74(1):253-62

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Mechanisms of acquired resistance to EGFR-tyrosine kinase inhibitor in Korean patients with lung cancer.

Ji W, Choi CM, Rho JK, Jang SJ, Park YS, Chun SM, Kim WS, Lee JS, Kim SW, Lee DH, Lee JC
BMC cancer 2013 Dec 27;13:606

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Cross-phosphorylation, signaling and proliferative functions of the Tyro3 and Axl receptors in Rat2 cells.

Brown JE, Krodel M, Pazos M, Lai C, Prieto AL
PloS one 2012;7(5):e36800

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Prolonged exposure to a Mer ligand in leukemia: Gas6 favors expression of a partial Mer glycoform and reveals a novel role for Mer in the nucleus.

Migdall-Wilson J, Bates C, Schlegel J, Brandão L, Linger RM, DeRyckere D, Graham DK
PloS one 2012;7(2):e31635

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Identification of Axl as a downstream effector of TGF-β1 during Langerhans cell differentiation and epidermal homeostasis.

Bauer T, Zagórska A, Jurkin J, Yasmin N, Köffel R, Richter S, Gesslbauer B, Lemke G, Strobl H
The Journal of experimental medicine 2012 Oct 22;209(11):2033-47

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Activation of the AXL kinase causes resistance to EGFR-targeted therapy in lung cancer.

Zhang Z, Lee JC, Lin L, Olivas V, Au V, LaFramboise T, Abdel-Rahman M, Wang X, Levine AD, Rho JK, Choi YJ, Choi CM, Kim SW, Jang SJ, Park YS, Kim WS, Lee DH, Lee JS, Miller VA, Arcila M, Ladanyi M, Moonsamy P, Sawyers C, Boggon TJ, Ma PC, Costa C, Taron M, Rosell R, Halmos B, Bivona TG
Nature genetics 2012 Jul 1;44(8):852-60

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Increased secretion of Gas6 by smooth muscle cells in human atherosclerotic carotid plaques.

Clauser S, Meilhac O, Bièche I, Raynal P, Bruneval P, Michel JB, Borgel D
Thrombosis and haemostasis 2012 Jan;107(1):140-9

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Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry.

Shimojima M, Ströher U, Ebihara H, Feldmann H, Kawaoka Y
Journal of virology 2012 Feb;86(4):2067-78

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Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for Mer tyrosine kinase-mediated phagocytosis of apoptotic cells.

Uehara H, Shacter E
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2522-30

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Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for Mer tyrosine kinase-mediated phagocytosis of apoptotic cells.

Uehara H, Shacter E
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2522-30

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Pharmacoproteomics of a metalloproteinase hydroxamate inhibitor in breast cancer cells: dynamics of membrane type 1 matrix metalloproteinase-mediated membrane protein shedding.

Butler GS, Dean RA, Tam EM, Overall CM
Molecular and cellular biology 2008 Aug;28(15):4896-914

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Axl/phosphatidylinositol 3-kinase signaling inhibits mineral deposition by vascular smooth muscle cells.

Collett GD, Sage AP, Kirton JP, Alexander MY, Gilmore AP, Canfield AE
Circulation research 2007 Mar 2;100(4):502-9

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Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.

Gould WR, Baxi SM, Schroeder R, Peng YW, Leadley RJ, Peterson JT, Perrin LA
Journal of thrombosis and haemostasis : JTH 2005 Apr;3(4):733-41

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Axl by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1.0 µg/mL of Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axl as an approximately 140 kDa glycoprotein (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Western Blot Shows Human Axl Specificity by Using Knockout Cell Line. Western blot shows lysates of A431 human epithelial carcinoma parental cell line and Axl knockout A431 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axl at approximately 150 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Axl in Human Stomach. Axl was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of epithelial cells in gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Axl in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Goat Anti-Human Axl Affinity-Purified Polyclonal Antibody (Catalog # AF154, filled histogram) or normal goat IgG control antibody (Catalog # AB-108C, open histogram) followed by APC-conjugated anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Axl Specificity is Shown by Flow Cytometry in Knockout Cell Line. Axl knockout A431 human epithelial carcinoma cell line was stained with Goat Anti-Human Axl Affinity-Purified Polyclonal Antibody (Catalog # AF154, filled histogram) or normal goat IgG control antibody (Catalog # AB-108C, open histogram) followed by APC-conjugated anti-Goat IgG Secondary Antibody (Catalog # F0108). No staining was observed in the Axl knockout A431 cell line. View our protocol for Staining Membrane-associated Proteins.