Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
Reference
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- Product number
- TA504923 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504923, RRID:AB_2622705
- Product name
- COMMD1 mouse monoclonal antibody, clone OTI1G7 (formerly 1G7)
- Antibody type
- Monoclonal
- Description
- COMMD1 mouse monoclonal antibody, clone OTI1G7 (formerly 1G7)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- COMMD1
- Isotype
- IgG
- Antibody clone number
- OTI1G7
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references Soluble Factors Secreted by Endothelial Cells Allow for Productive and Latent HIV-1 Infection in Resting CD4+ T Cells.
Morris JH 3rd, Nguyen T, Nwadike A, Geels ML, Kamp DL, Kim BR, Boyer JD, Shen A
AIDS research and human retroviruses 2017 Feb;33(2):110-120
AIDS research and human retroviruses 2017 Feb;33(2):110-120
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COMMD1 (RC205614, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COMMD1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-COMMD1 mouse monoclonal antibody (TA504923) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY COMMD1(RC205614).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-COMMD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504923)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-COMMD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504923)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-COMMD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504923)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC205614 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-COMMD1 antibody(TA504923), and then analyzed by flow cytometry.
- Validation comment
- FC