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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MAB7526 - Provider product page
- Provider
- R&D Systems
- Product name
- Human COMMD1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human COMMD1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Attractin or rhCaspr1 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q8N668- Isotype
- IgG
- Antibody clone number
- 762203
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The COMMD Family Regulates Plasma LDL Levels and Attenuates Atherosclerosis Through Stabilizing the CCC Complex in Endosomal LDLR Trafficking.
COMMD5/HCaRG Hooks Endosomes on Cytoskeleton and Coordinates EGFR Trafficking.
Fedoseienko A, Wijers M, Wolters JC, Dekker D, Smit M, Huijkman N, Kloosterhuis N, Klug H, Schepers A, Willems van Dijk K, Levels JHM, Billadeau DD, Hofker MH, van Deursen J, Westerterp M, Burstein E, Kuivenhoven JA, van de Sluis B
Circulation research 2018 Jun 8;122(12):1648-1660
Circulation research 2018 Jun 8;122(12):1648-1660
COMMD5/HCaRG Hooks Endosomes on Cytoskeleton and Coordinates EGFR Trafficking.
Campion CG, Zaoui K, Verissimo T, Cossette S, Matsuda H, Solban N, Hamet P, Tremblay J
Cell reports 2018 Jul 17;24(3):670-684.e7
Cell reports 2018 Jul 17;24(3):670-684.e7
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human COMMD1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line, HepG2 human hepatocellular carcinoma cell line, and human placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human COMMD1 Monoclonal Antibody (Catalog # MAB7526) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for COMMD1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- COMMD1 in U2OS Human Cell Line. COMMD1 was detected in immersion fixed U2OS human osteosarcoma cell line using Mouse Anti-Human COMMD1 Monoclonal Antibody (Catalog # MAB7526) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
