LS-C774102

antibody from LSBio

Targeting: GABRE

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C774102

Provider

LSBio

Product name

GABRE Antibody (clone S413-67) LS-C774102

Antibody type

Monoclonal

Description

Protein G purified

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

S413-67

Storage

Store at -20°C.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot analysis of Rat Brain showing detection of ~60 kDa GABA A Receptor Epsilon protein using Mouse Anti-GABA A Receptor Epsilon Monoclonal Antibody, Clone S413-67. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 10 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-GABA A Receptor Epsilon Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~60 kDa.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Epsilon Monoclonal Antibody, Clone S413-67. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Epsilon Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Synapse, Postsynaptic Cell Membrane, Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GABA-A Receptor Epsilon Antibody. (D) Composite.