CABT-B9244

antibody from Creative Diagnostics

Targeting: SLC9A1 APNH, NHE1, PPP1R143

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Antibody Data

Product number

CABT-B9244

Provider

Creative Diagnostics

Product name

Mouse anti-Rat NHE monoclonal antibody, clone 65/OIF

Antibody type

Monoclonal

Description

The extrusion of H+ in exchange for extracellular Na+ is important for many cellular processes, such as pH homeostasis, volume regulation, and transepithelial ion and water transport. Na+/H+ Exchangers (NHE) are integral membrane proteins that mediate electroneutral exchange of one Na+ ion for one H+ ion. Six NHE forms, NHE-1 thru -6, have been identified. NHE-1 and NHE-6 are widely expressed, while the other NHE forms have restricted expression. The common structure of all NHE forms includes 10-12 N-terminal membrane (M) spanning regions, a conserved M6 and M7 region that may participate in ion transport, and a large C-terminal cytoplasmic region that may be involved in the regulation of ion exchange activity. NHE-1, for example, contains 12 M regions plus domains for volume sensitivity, calmodulin-binding, CHP-binding, and PKC phosphorylation in the cytoplasmic region. Regulation of NHE-1 ion exchange activity may occur through phosphoinositide binding, as well as PKC- and PKA-dependent signaling pathways. Mutation of NHE-1 in mice causes neuronal death in the cerebellum and brainstem, leading to ataxia and seizures. Thus, NHE-1 is a ubiquitous NHE that is essential for normal brain function.

Reactivity

Human, Mouse, Rat, Canine

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

65/OIF

Vial size

50ug, 150ug

Concentration

250 Ã×g/ml

Storage

Store undiluted at -20°C.