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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ANX-010-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Na+/H+ Exchanger 1 (NHE-1) (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membranes (lanes 1 and 6), mouse brain lysate (lanes 2 and 7), human MCF-7 breast adenocarcinoma cells (lanes 3 and 8), human U-87 MG glioblastoma cells (lanes 4 and 9) and human THP-1 acute monocytic leukemia cells (lanes 5 and 10): - 1-5. Anti-Na+/H+Exchanger 1 (NHE-1) (extracellular) Antibody (#ANX-010), (1:200).6-10. Anti-Na+/H+Exchanger 1 (NHE-1) (extracellular) Antibody , preincubated with Na+/H+ Exchanger 1/NHE-1 (extracellular) Blocking Peptide (#BLP-NX010).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membranes (lanes 1 and 6), mouse brain lysate (lanes 2 and 7), human MCF-7 breast adenocarcinoma cells (lanes 3 and 8), human U-87 MG glioblastoma cells (lanes 4 and 9) and human THP-1 acute monocytic leukemia cells (lanes 5 and 10): - 1-5. Anti-Na+/H+Exchanger 1 (NHE-1) (extracellular) Antibody (#ANX-010), (1:200).6-10. Anti-Na+/H+Exchanger 1 (NHE-1) (extracellular) Antibody , preincubated with Na+/H+ Exchanger 1/NHE-1 (extracellular) Blocking Peptide (#BLP-NX010).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of NHE1 in human MCF-7 cells - Cell surface detection of NHE1 in live intact human MCF-7 breast adenocarcinoma cells. A. Extracellular labeling of cells with Anti-Na+/H+Exchanger 1 (NHE-1) (extracellular) Antibody (#ANX-010), (1:25), followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Live view of the cells. C. Merge of the two pictures.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of NHE1 in mouse substantia nigra pars compacta - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Na+/H+Exchanger 1 (NHE-1) (extracellular) Antibody (#ANX-010), (1:200), followed by goat- Anti-rabbit-AlexaFluor-488. NHE1 immunoreactivity (green) appears in neuronal profiles (arrows). Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of NHE1 by indirect flow cytometry in live intact human MEG-01 megakaryocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Na+/H+ Exchanger 1 (NHE-1) (extracellular) Antibody (#ANX-010), (2.5μg) + goat- Anti-rabbit-FITC.
