PA5-89830

antibody from Invitrogen Antibodies

Targeting: ASCL4 bHLHa44, HASH4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-89830

Provider

Invitrogen Antibodies

Product name

ASCL4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MMETRKPAER LALPYSLRTA PLGVPGTLPG LPRRDPLRVA LRLDAACWEW ARSGCARGWQ YLPVPLDSAF EPAFLRKRNE RERQRVRCVN EGYARLRDHL PRELADKRLS KVETLRAAID YIKHLQELLE RQAWGLEGAA GAVPQRRAEC NSDGESKASS APSPSSEPEE GG; Positive Samples: A431, A549, SGC-7901, 293T, MCF7; Cellular Location: Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.68 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using ASCL4 Polyclonal antibody (Product # PA5-89830) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 5s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ASCL4 in extracts of various cell lines using ASCL4 Polyclonal Antibody (Product # PA5-89830) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ASCL4 in paraffin-embedded human lung cancer using ASCL4 Polyclonal Antibody (Product # PA5-89830) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ASCL4 in paraffin-embedded human appendix using ASCL4 Polyclonal Antibody (Product # PA5-89830) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ASCL4 in paraffin-embedded human breast cancer using ASCL4 Polyclonal Antibody (Product # PA5-89830) at a dilution of 1:100.