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LearnNB100-79793
antibody from Novus Biologicals
Targeting: SPAG9
CT89, FLJ13450, FLJ14006, FLJ26141, FLJ34602, HLC4, HSS, JIP-4, JIP4, JLP, KIAA0516, MGC117291, MGC14967, MGC74461, PHET, PIG6, SYD1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-79793 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-79793, RRID:AB_1109079
- Product name
- Rabbit Polyclonal SPAG9 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPAG9 Antibody [NB100-79793] - Nuclear extract (NE, 20 ug) from HeLa cells. Antibody used at 0.3 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPAG9 Antibody [NB100-79793] - Detection of human and mouse JLP/SPAG9 by western blot. Samples: Whole cell lysate (10 ug) from GaMG, HEK293T, A-549, Hep-G2, U2OS, HeLa, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JLP antibody NB100-79793 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SPAG9 Antibody [NB100-79793] - Detection of human JLP/SPAG9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JLP antibody NB100-79793 (lot 4) used for IP at 6 ug per reaction. JLP was also immunoprecipitated by a previous lot of this antibody (lot 3) and rabbit anti-JLP antibody NB100-79794. For blotting immunoprecipitated JLP, NB100-79793 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: SPAG9 Antibody [NB100-79793] - Sample: FFPE section of human testis (left) and testicular seminoma (right). Antibody: Affinity purified rabbit anti-JLP used at a dilution of 1:5,000 (0.2 ug/ml) and 1:1,000 (1ug/ml). Detection: DAB
