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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [15]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- 10758-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#10758-1-AP, RRID:AB_2115593
- Product name
- HADHA antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated HADHA antibody (Cat. #10758-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references The Impaired Bioenergetics of Diabetic Cardiac Microvascular Endothelial Cells.
Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.
Rescue of myocardial energetic dysfunction in diabetes through the correction of mitochondrial hyperacetylation by honokiol.
High-Dose Dexamethasone Manipulates the Tumor Microenvironment and Internal Metabolic Pathways in Anti-Tumor Progression.
The protein acetylase GCN5L1 modulates hepatic fatty acid oxidation activity via acetylation of the mitochondrial β-oxidation enzyme HADHA.
Establishment of a novel hepatic steatosis cell model by Cas9/sgRNA-mediated DGKθ gene knockout.
Succinate accumulation impairs cardiac pyruvate dehydrogenase activity through GRP91-dependent and independent signaling pathways: Therapeutic effects of ginsenoside Rb1.
Acetylation of mitochondrial proteins by GCN5L1 promotes enhanced fatty acid oxidation in the heart.
Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization.
Effect of high-fat diet on hepatic proteomics of hamsters.
DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.
The trifunctional protein mediates thyroid hormone receptor-dependent stimulation of mitochondria metabolism.
Pressure load: the main factor for altered gene expression in right ventricular hypertrophy in chronic hypoxic rats.
Men supplemented with 17beta-estradiol have increased beta-oxidation capacity in skeletal muscle.
Women have higher protein content of beta-oxidation enzymes in skeletal muscle than men.
Zhang H, Shen Y, Kim IM, Weintraub NL, Tang Y
Frontiers in endocrinology 2021;12:642857
Frontiers in endocrinology 2021;12:642857
Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.
Wang W, Hu X, Xia Z, Liu Z, Zhong Z, Lu Z, Liu A, Ye S, Cao Q, Wang Y, Zhu F, Ye Q
Oxidative medicine and cellular longevity 2020;2020:5849794
Oxidative medicine and cellular longevity 2020;2020:5849794
Rescue of myocardial energetic dysfunction in diabetes through the correction of mitochondrial hyperacetylation by honokiol.
Kerr M, Miller JJ, Thapa D, Stiewe S, Timm KN, Aparicio CNM, Scott I, Tyler DJ, Heather LC
JCI insight 2020 Sep 3;5(17)
JCI insight 2020 Sep 3;5(17)
High-Dose Dexamethasone Manipulates the Tumor Microenvironment and Internal Metabolic Pathways in Anti-Tumor Progression.
Xu L, Xia H, Ni D, Hu Y, Liu J, Qin Y, Zhou Q, Yi Q, Xie Y
International journal of molecular sciences 2020 Mar 7;21(5)
International journal of molecular sciences 2020 Mar 7;21(5)
The protein acetylase GCN5L1 modulates hepatic fatty acid oxidation activity via acetylation of the mitochondrial β-oxidation enzyme HADHA.
Thapa D, Wu K, Stoner MW, Xie B, Zhang M, Manning JR, Lu Z, Li JH, Chen Y, Gucek M, Playford MP, Mehta NN, Harmon D, O'Doherty RM, Jurczak MJ, Sack MN, Scott I
The Journal of biological chemistry 2018 Nov 16;293(46):17676-17684
The Journal of biological chemistry 2018 Nov 16;293(46):17676-17684
Establishment of a novel hepatic steatosis cell model by Cas9/sgRNA-mediated DGKθ gene knockout.
Zhang J, Zhao J, Zheng X, Cai K, Mao Q, Xia H
Molecular medicine reports 2018 Feb;17(2):2169-2176
Molecular medicine reports 2018 Feb;17(2):2169-2176
Succinate accumulation impairs cardiac pyruvate dehydrogenase activity through GRP91-dependent and independent signaling pathways: Therapeutic effects of ginsenoside Rb1.
Li J, Yang YL, Li LZ, Zhang L, Liu Q, Liu K, Li P, Liu B, Qi LW
Biochimica et biophysica acta. Molecular basis of disease 2017 Nov;1863(11):2835-2847
Biochimica et biophysica acta. Molecular basis of disease 2017 Nov;1863(11):2835-2847
Acetylation of mitochondrial proteins by GCN5L1 promotes enhanced fatty acid oxidation in the heart.
Thapa D, Zhang M, Manning JR, Guimarães DA, Stoner MW, O'Doherty RM, Shiva S, Scott I
American journal of physiology. Heart and circulatory physiology 2017 Aug 1;313(2):H265-H274
American journal of physiology. Heart and circulatory physiology 2017 Aug 1;313(2):H265-H274
Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization.
Yang W, Nagasawa K, Münch C, Xu Y, Satterstrom K, Jeong S, Hayes SD, Jedrychowski MP, Vyas FS, Zaganjor E, Guarani V, Ringel AE, Gygi SP, Harper JW, Haigis MC
Cell 2016 Nov 3;167(4):985-1000.e21
Cell 2016 Nov 3;167(4):985-1000.e21
Effect of high-fat diet on hepatic proteomics of hamsters.
Liao CC, Lin YL, Kuo CF
Journal of agricultural and food chemistry 2015 Feb 18;63(6):1869-81
Journal of agricultural and food chemistry 2015 Feb 18;63(6):1869-81
DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.
Richter-Dennerlein R, Korwitz A, Haag M, Tatsuta T, Dargazanli S, Baker M, Decker T, Lamkemeyer T, Rugarli EI, Langer T
Cell metabolism 2014 Jul 1;20(1):158-71
Cell metabolism 2014 Jul 1;20(1):158-71
The trifunctional protein mediates thyroid hormone receptor-dependent stimulation of mitochondria metabolism.
Chocron ES, Sayre NL, Holstein D, Saelim N, Ibdah JA, Dong LQ, Zhu X, Cheng SY, Lechleiter JD
Molecular endocrinology (Baltimore, Md.) 2012 Jul;26(7):1117-28
Molecular endocrinology (Baltimore, Md.) 2012 Jul;26(7):1117-28
Pressure load: the main factor for altered gene expression in right ventricular hypertrophy in chronic hypoxic rats.
Baandrup JD, Markvardsen LH, Peters CD, Schou UK, Jensen JL, Magnusson NE, Ørntoft TF, Kruhøffer M, Simonsen U
PloS one 2011 Jan 5;6(1):e15859
PloS one 2011 Jan 5;6(1):e15859
Men supplemented with 17beta-estradiol have increased beta-oxidation capacity in skeletal muscle.
Maher AC, Akhtar M, Tarnopolsky MA
Physiological genomics 2010 Aug;42(3):342-7
Physiological genomics 2010 Aug;42(3):342-7
Women have higher protein content of beta-oxidation enzymes in skeletal muscle than men.
Maher AC, Akhtar M, Vockley J, Tarnopolsky MA
PloS one 2010 Aug 6;5(8):e12025
PloS one 2010 Aug 6;5(8):e12025
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10758-1-AP(HADHA antibody) at dilution of 1:1000
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using HADHA antibody 10758-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-HADHA (IP:10758-1-AP, 4ug; Detection:10758-1-AP 1:200) with HEK-293 cells lysate 2500ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon cancer using 10758-1-AP(HADHA antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
