PAB20813

antibody from Abnova Corporation

Targeting: HADHA GBP, LCEH, LCHAD, MTPA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20813

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20813, RRID:AB_10963472

Product name

HADHA polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant HADHA.

Antigen sequence

IEYLEEVAITFAKGLADKKISPKRDKGLVEKLTAY
AMTIPFVRQQVYKKVEEKVRKQTKGLYPAPLKIID
VVKTGIEQGSDAGYLCESQKFGELVMTKESKALMG
LYHGQVLCKKNKFGAPQKDVKHLAILGAGLMGAGI
AQVSVDK


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with HADHA polyclonal antibody (Cat # PAB20813) at 1:250-1:500 dilution.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of cell lysates with HADHA polyclonal antibody (Cat # PAB20813) at 1:250-1:500 dilution.Lane 1 : NIH/3T3Lane 2 : NBT-II

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with HADHA polyclonal antibody (Cat # PAB20813) at 1-4 ug/mL dilution shows positivity in mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human small intestine with HADHA polyclonal antibody (Cat # PAB20813) shows strong cytoplasmic positivity in glandular cells at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)