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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ABIN453085 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Hydroxyacyl-Coenzyme A Dehydrogenase/3-Ketoacyl-Coenzyme A Thiolase/enoyl-Coenzyme A Hydratase (Trifunctional Protein), alpha Subunit (HADHA) (C-Term), (AA 734-763) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 734~763 amino acids from the C-terminal region of human HADHA
- Description
- Protein A Chromatography, followed by peptide affinity purification
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Epitope
- C-Term,AA 734-763
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references The molecular basis of pediatric long chain 3-hydroxyacyl-CoA dehydrogenase deficiency associated with maternal acute fatty liver of pregnancy.
Sims HF, Brackett JC, Powell CK, Treem WR, Hale DE, Bennett MJ, Gibson B, Shapiro S, Strauss AW
Proceedings of the National Academy of Sciences of the United States of America 1995 Jan 31;92(3):841-5
Proceedings of the National Academy of Sciences of the United States of America 1995 Jan 31;92(3):841-5
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of HADHA Antibody (C-term) Cat.-No AP17454PU-N in A2058, A375, NCI-H460, Ramos cell line lysates (35ug/lane). HADHA (arrow) was detected using the purified Pab
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HADHA Antibody (C-term) Cat.-No AP17454PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Ramos cells using HADHA Antibody (C-term) Cat.-No AP17454PU-N (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
