10758-1-AP

antibody from Invitrogen Antibodies

Targeting: HADHA GBP, LCEH, LCHAD, MTPA

Provider product page for 10758-1-AP

Western blot

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [0]
Comments [0]
Validations
Western blot [11]
Immunocytochemistry [4]
Immunohistochemistry [11]
Flow cytometry [1]
Other assay [1]

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Product number: 10758-1-AP - Provider product page
Provider: Invitrogen Antibodies
Product name: HADHA Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Description: Immunogen sequence: LMGLYHGQV LCKKNKFGAP QKDVKHLAIL GAGLMGAGIA QVSVDKGLKT ILKDATLTAL DRGQQQVFKG LNDKVKKKAL TSFERDSIFS NLTGQLDYQG FEKADMVIEA VFEDLSLKHR VLKEVEAVIP DHCIFASNTS ALPISEIAAV SKRPEKVIGM HYFSPVDKMQ LLEIITTEKT SKDTSASAVA VGLKQGKVII VVKDGPGFYT TRCLAPMMSE VIRILQEGVD PKKLDSLTTS FGFPVGAATL VDEVGVDVAK HVAEDLGKVF GERFGGGNPE LLTQMVSKGF LGRKSGKGFY IYQEGVKRKD LNSDMDSILA SLKLPPKSEV SSDEDIQFRL VTRFVNEAVM CLQEGILATP AEGDIGAVFG LGFPPCLGGP FRFVDLYGAQ KIVDRLKKYE AAYGKQFTPC QLLADHANSP NKKFYQ (339-763 aa encoded by BC009235)
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 150 µL
Concentration: 0.2 mg/mL
Storage: -20°C

HADHA protein structure - 10758-1-AP shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Various lysates were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Various lysates were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: MCF7 cells were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Rat kidney tissue were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: WB result of HADHA antibody (10758-1-AP, 1:4000) with si-control and si-HADHA transfected HeLa cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse heart tissue were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Jurkat cells were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 10758-1-AP (HADHA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human placenta using 10758-1-AP (HADHA antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human ovary using 10758-1-AP (HADHA antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human placenta using 10758-1-AP (HADHA antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human testis using 10758-1-AP (HADHA antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human testis using 10758-1-AP (HADHA antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human spleen using 10758-1-AP (HADHA antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human spleen using 10758-1-AP (HADHA antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human lung using 10758-1-AP (HADHA antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human lung using 10758-1-AP (HADHA antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human ovary using 10758-1-AP (HADHA antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human colon cancer using 10758-1-AP (HADHA antibody) at dilution of 1:100 (under 40x lens).