F54313

antibody from NSJ Bioreagents

Targeting: PLA2G7 LDL-PLA2, PAFAH

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54313

Provider

NSJ Bioreagents

Product name

PLA2G7 Antibody / Lp-PLA2 / PAFAH

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 µl, 0.4 µl

Storage

Aliquot the Lp-PLA2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) liver, 2) lung, 3) spleen and 4) plasma lysate with Lp-PLA2 antibody. Expected molecular weight: 45-67 kDa depending on glycosylation level.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human HL60 cell lysate with Lp-PLA2 antibody. Expected molecular weight: 45-67 kDa depending on glycosylation level.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human tonsil tissue with Lp-PLA2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human tonsil tissue with Lp-PLA2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human colon tissue with Lp-PLA2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human HL60 cells with Lp-PLA2 antibody; Blue=isotype control, Green= Lp-PLA2 antibody.