FNab04402

antibody from FineTest Biotech

Targeting: ISCU hnifU, ISU2, NIFUN

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

FNab04402

Provider

FineTest Biotech

Product name

ISCU antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Scaffold protein for the de novo synthesis of iron-sulfur(Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins(PubMed:11060020). First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH(By similarity).

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

iron-sulfur cluster scaffold homolo
g(E. coli)


Epitope

IgG

Isotype

IgG

Vial size

100¦Ìg

Concentration

Immunogen affinity purified

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20¡æ for 12 months(Avoid repeated freeze / thaw cycles.)

Handling

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20¡æ for 12 months(Avoid repeated freeze / thaw cycles.)