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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB4745 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4745, RRID:AB_1576980
- Product name
- MAGEA3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MAGEA3.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The cancer/testis antigen melanoma-associated antigen-A3/A6 is a novel target of fibroblast growth factor receptor 2-IIIb through histone H3 modifications in thyroid cancer.
Kondo T, Zhu X, Asa SL, Ezzat S
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Aug 15;13(16):4713-20
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Aug 15;13(16):4713-20
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The MAGEA3 polyclonal antibody (Cat # PAB4745) is used in Western blot to detect MAGEA3 in Jurkat cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human breast cancer tissue withMAGEA3 polyclonal antibody (Cat # PAB4745). Immunohistochemistry of formalin-fixed, paraffin-embedded tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells using MAGEA3 polyclonal antibody (Cat # PAB4745) (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis
- Validation comment
- Flow Cytometry
