628801

antibody from BioLegend

Targeting: CETN2 CALT, CEN2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

628801

Provider

BioLegend

Proper citation

BioLegend Cat#628801, RRID:AB_2077385

Product name

Purified anti-Centrin 2 (Caltractin)

Antibody type

Polyclonal

Antigen

Recombinant centrin 2 protein

Description

Centrin 2, also known as caltractin, EF-hand protein 2, and 20 kD calcium-binding protein, is a calcium-binding protein that contains 4 EF-hand domains. Centrin 2 is highly expressed in the retina and testis, also expressed in liver, skeletal muscle and cardiac muscle. The centrin-2 protein plays a critical function in normal cell division,and is known to associate with the centrosomes, mitotic spindle poles, and basal bodies. Centrin 2 has a fundamental role in the structure and function of the microtubule-organizing center and has been shown to associate with the RAD23B, SFI1, and Xeroderma pigmentosum group C complementing proteins. The Poly6288 antibody recognizes human centrin 2 and has been shown to be useful for Western blotting.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

50 µl

Storage

Upon receipt, store frozen at -20° C.

References

Submitted references

Defective nucleotide excision repair with normal centrosome structures and functions in the absence of all vertebrate centrins.

Dantas TJ, Wang Y, Lalor P, Dockery P, Morrison CG
The Journal of cell biology 2011 Apr 18;193(2):307-18

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-centrin 2 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

Conjugate

Unconjugated