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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-21775 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Coronin 3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2.
- Concentration
- 1 mg/mL
Submitted references MICAL2 enhances branched actin network disassembly by oxidizing Arp3B-containing Arp2/3 complexes.
Novel Potential Biomarkers Associated With Epithelial to Mesenchymal Transition and Bladder Cancer Prognosis Identified by Integrated Bioinformatic Analysis.
Galloni C, Carra D, Abella JVG, Kjær S, Singaravelu P, Barry DJ, Kogata N, Guérin C, Blanchoin L, Way M
The Journal of cell biology 2021 Aug 2;220(8)
The Journal of cell biology 2021 Aug 2;220(8)
Novel Potential Biomarkers Associated With Epithelial to Mesenchymal Transition and Bladder Cancer Prognosis Identified by Integrated Bioinformatic Analysis.
Wang C, Yang Y, Yin L, Wei N, Hong T, Sun Z, Yao J, Li Z, Liu T
Frontiers in oncology 2020;10:931
Frontiers in oncology 2020;10:931
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Coronin 3 using 30 µg of A) A431 and B) H1299 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Coronin 3 polyclonal antibody (Product # PA5-21775) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Coronin 3 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Coronin 3 Polyclonal Antibody (Product # PA5-21775) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Coronin 3 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Coronin 3 Polyclonal Antibody (Product # PA5-21775) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Coronin 3 in methanol-fixed HeLa cells using a Coronin 3 polyclonal antibody (Product # PA5-21775) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using Coronin 1C antibody (Product # PA5-21775) at 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using Coronin 1C antibody (Product # PA5-21775) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human Sarcoma meta to brain Blood Vessel, using CORO1C (Product # PA5-21775) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 8. Coronin 1C recruits MICAL2 to vaccinia-induced actin tails. (A) Image stills from Video 8 and Video 9 showing coronin 1C is required to recruit GFP-MICAL2 (green) to vaccinia-induced actin tails visualized with LifeAct-iRFP (magenta). Scale bars = 5 um (main image) and 2.5 um (insert). The immunoblot shows the level of coronin 1C in HeLa cells treated with coronin 1C siRNA, while the graph shows the percentage of actin tails recruiting GFP-MICAL2 following coronin 1C knockdown. The number of cells and actin tails analyzed is indicated. Student's t test was used to determine statistical significance for n = 4 independent experiments. (B) Immunoblot analysis of GFP-Trap pulldowns on HeLa cells stably expressing the indicated GFP-tagged protein demonstrates coronin 1C (Coro1C) associates with MICAL2, but not MICAL1.
