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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX118473 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX118473, RRID:AB_11176191
- Product name
- EXOSC5 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HeLa whole cell and nuclear extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with EXOSC5 antibody (GTX118473) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using EXOSC5(GTX118473) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EXOSC5 antibody detects EXOSC5 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: EXOSC5 protein stained by EXOSC5 antibody (GTX118473) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using EXOSC5(GTX118473) antibody at 1:500 dilution.
