Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NB100-2300 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2300, RRID:AB_10003036
- Product name
- Rabbit Polyclonal PARC Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PARC Antibody [NB100-2300] - Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PARC/H7-AP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PARC Antibody [NB100-2300] - Detection of Human PARC/H7-AP1 on HeLa whole cell lysate using NB100-2300. Normal rabbit IgG was used as a control for immunoprecipitation (IP Control).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PARC Antibody [NB100-2300] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PARC/H7-AP1 antibody (lot was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.