Explore
Validate
Learn
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB44782-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CEACAM-7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CEACAM-7 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q14002- Isotype
- IgG
- Antibody clone number
- 962720
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Human CEACAM-7 ELISA Standard Curve. Recombinant Human CEACAM-7 protein was serially diluted 2-fold and captured by Mouse Anti-Human CEACAM-7 Monoclonal Antibody (Catalog # MAB44782) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human CEACAM-7 Monoclonal Antibody (Catalog # MAB44781) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CEACAM-7 in Human Colon Cancer Tissue. CEACAM-7 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human CEACAM-7 Monoclonal Antibody (Catalog # MAB44782) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CEACAM-7 in HEK293 Human Cell Line Transfected with Human CEACAM-7 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human CEACAM-7 and eGFP was stained with either (A) Mouse Anti-Human CEACAM-7 Monoclonal Antibody (Catalog # MAB44782) or (B) Mouse IgG1 Isotype Control(Catalog # MAB002) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
