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- Antigen structure
- References [3]
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- Western blot [1]
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- Product number
- PAB16897 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB16897, RRID:AB_10678179
- Product name
- CENPQ polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against full length recombinant CENPQ.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references CENP-O class proteins form a stable complex and are required for proper kinetochore function.
The human CENP-A centromeric nucleosome-associated complex.
The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres.
Hori T, Okada M, Maenaka K, Fukagawa T
Molecular biology of the cell 2008 Mar;19(3):843-54
Molecular biology of the cell 2008 Mar;19(3):843-54
The human CENP-A centromeric nucleosome-associated complex.
Foltz DR, Jansen LE, Black BE, Bailey AO, Yates JR 3rd, Cleveland DW
Nature cell biology 2006 May;8(5):458-69
Nature cell biology 2006 May;8(5):458-69
The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres.
Okada M, Cheeseman IM, Hori T, Okawa K, McLeod IX, Yates JR 3rd, Desai A, Fukagawa T
Nature cell biology 2006 May;8(5):446-57
Nature cell biology 2006 May;8(5):446-57
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using CENPQ polyclonal antibody (Cat # PAB16897) shows detection of endogenous CENPQ in a HeLa whole cell lysate (Lane 1, arrowhead). The blot was incubated for 1.5 hours at room temperature using the primary antibody diluted to 0.5 ug/mL , followed by washes and incubation with to the secondary antibody.Lane 1 : Lysates from HeLa cells transfected with control sh-virus.Lane 2 : Lysates from HeLa cells transfected with CENPQ sh-virus.Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.
