AP09161PU-N

antibody from Acris Antibodies GmbH

Targeting: CENPQ C6orf139, CENP-Q, FLJ10545

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP09161PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09161PU-N, RRID:AB_2035349

Product name

anti CENP-Q

Antibody type

Polyclonal

Antigen

Prepared from whole rabbit serum produced by repeated immunizations with full-length human CENP-Q recombinant protein

Reactivity

Human, Mouse, Rat, Bovine, Canine, Porcine

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.15 mg/ml (by UV absorbance at 280 nm)

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescence microscopy using protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in HeLa whole cell lysate. Primary antibody was used at 1:100 followed by secondary antibody diluted 1:150. Red punctate anti-CENP-Q signal colocalizes in overlay images with green punctate anti-CREST signals at the kinetochores (attached points of sister chromatids). Visible are colocalized CENP-Q and CREST signal at various stages of the cell cycle as indicated from interphase to the end of mitosis. Nuclei are counter stained with bisbenzimide.