Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- 14259-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14259-1-AP, RRID:AB_2198222
- Product name
- Syntaxin 12 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated Syntaxin 12 antibody (Cat. #14259-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway.
Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.
Syntaxin 13, a genetic modifier of mutant CHMP2B in frontotemporal dementia, is required for autophagosome maturation.
Lv Y, Wang X, Li X, Xu G, Bai Y, Wu J, Piao Y, Shi Y, Xiang R, Wang L
PLoS biology 2020 Nov;18(11):e3000872
PLoS biology 2020 Nov;18(11):e3000872
Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.
Malik N, Nirujogi RS, Peltier J, Macartney T, Wightman M, Prescott AR, Gourlay R, Trost M, Alessi DR, Karapetsas A
The Biochemical journal 2019 Oct 30;476(20):3081-3107
The Biochemical journal 2019 Oct 30;476(20):3081-3107
Syntaxin 13, a genetic modifier of mutant CHMP2B in frontotemporal dementia, is required for autophagosome maturation.
Lu Y, Zhang Z, Sun D, Sweeney ST, Gao FB
Molecular cell 2013 Oct 24;52(2):264-71
Molecular cell 2013 Oct 24;52(2):264-71
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse brain tissue were subjected to SDS PAGE followed by western blot with 14259-1-AP(STX12 antibody) at dilution of 1:1000
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using STX12 antibody 14259-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human lung cancer using 14259-1-AP(STX12 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human lung cancer using 14259-1-AP(STX12 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The Syntaxin 12 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human Syntaxin 12. This antibody recognizes human,mouse,rat antigen. The Syntaxin 12 antibody has been validated for the following applications: ELISA, WB, IHC, IF, FC analysis.