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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunohistochemistry [1]
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- Product number
- MAB23971 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CRISP-3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CRISP-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 50-100% cross-reactivity with recombinant human CRISP-2 is observed.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Eukaryotic expression, purification and structure/function analysis of native, recombinant CRISP3 from human and mouse.
Expression microarray analysis identifies novel epithelial-derived protein markers in eosinophilic esophagitis.
Volpert M, Mangum JE, Jamsai D, D'Sylva R, O'Bryan MK, McIntyre P
Scientific reports 2014 Feb 27;4:4217
Scientific reports 2014 Feb 27;4:4217
Expression microarray analysis identifies novel epithelial-derived protein markers in eosinophilic esophagitis.
Matoso A, Mukkada VA, Lu S, Monahan R, Cleveland K, Noble L, Mangray S, Resnick MB
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2013 May;26(5):665-76
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2013 May;26(5):665-76
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- CRISP-3 in Human Prostate. CRISP-3 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human CRISP-3 Monoclonal Antibody (Catalog # MAB23971) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
