PA5-88885

antibody from Invitrogen Antibodies

Targeting: PGK2 PGK-2, PGKPS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-88885

Provider

Invitrogen Antibodies

Product name

PGK2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: YTFLKVLNNM EIGASLFDEE GAKIVKDIMA KAQKNGVRIT FPVDFVTGDK FDENAQVGKA TVASGISPGW MGLDCGPESN KNHAQVVAQA RLIVWNGPLG VFEWDAFAKG TKALMDEIV; Positive Samples: U-87MG, Jurkat, HeLa, HepG2, Mouse testis, Mouse brain, Mouse heart, Rat testis, Rat brain; Cellular Location: Cytoplasm

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.53 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using PGK2 Polyclonal antibody (Product # PA5-88885) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 5s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PGK2 in extracts of various cell lines using PGK2 Polyclonal Antibody (Product # PA5-88885) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PGK2 in extracts of various cell lines using PGK2 Polyclonal Antibody (Product # PA5-88885) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PGK2 was performed in C6 cells using PGK2 Polyclonal Antibody (Product # PA5-88885) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PGK2 was performed in L929 cells using PGK2 Polyclonal Antibody (Product # PA5-88885) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PGK2 in paraffin-embedded human mammary cancer using PGK2 Polyclonal Antibody (Product # PA5-88885) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PGK2 in paraffin-embedded rat testis using PGK2 Polyclonal Antibody (Product # PA5-88885) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PGK2 in paraffin-embedded mouse testis using PGK2 Polyclonal Antibody (Product # PA5-88885) at a dilution of 1:100.