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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00003131-M04 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003131-M04, RRID:AB_463768
- Product name
- HLF monoclonal antibody (M04), clone M2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant HLF.
- Antigen sequence
MEKMSRPLPLNPTFIPPPYGVLRSLLENPLKLPLH
HEDAFSKDKDKEKKLDDESNSPTVPQSAFLGPTLW
DKTLPYDGDTFQLEYMDLEEFLSENGIPPSPSQHD
HSPHPPGLQPASSAAPSVMDLSSRASAPLHPGIPS
PNCMQSPIRPGQLLPANRNTPSPIDPDTIQVPVGY
EPDPADLALSSIPGQEMFDPRKRKFSEEELKPQPM
IKKARKVFIPDDLKDDKYWARRRKNNMAAKRSRDA
RRLKENQIAIRASFLEKENSALRQEVADLRKELGK
CKNILAKYEARHGPL- Isotype
- IgG
- Antibody clone number
- M2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Circadian clock-controlled intestinal expression of the multidrug-resistance gene mdr1a in mice.
Murakami Y, Higashi Y, Matsunaga N, Koyanagi S, Ohdo S
Gastroenterology 2008 Nov;135(5):1636-1644.e3
Gastroenterology 2008 Nov;135(5):1636-1644.e3
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of HLF expression in transfected 293T cell line by HLF monoclonal antibody (M04), clone M2.Lane 1: HLF transfected lysate(33.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to HLF on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
