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LearnAF4675
antibody from R&D Systems
Targeting: CRISP1
AEGL1, ARP, CRISP-1, HSCRISP1D, HSCRISP1G, HUMARP
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF4675 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CRISP-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse CRISP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CRISP-3 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q03401- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse CRISP-1 by Western Blot. Western blot shows lysates of mouse testis tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse CRISP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4675) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CRISP-1 at approximately 25-29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CRISP-1 in Mouse Epididymus. CRISP-1 was detected in immersion fixed frozen sections of mouse epididymus using Goat Anti-Mouse CRISP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4675) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
