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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- TA504424 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504424, RRID:AB_11128421
- Product name
- SPG7 mouse monoclonal antibody, clone OTI1C1 (formerly 1C1)
- Antibody type
- Monoclonal
- Description
- SPG7 mouse monoclonal antibody, clone OTI1C1 (formerly 1C1)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SPG7
- Isotype
- IgG
- Antibody clone number
- OTI1C1
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Proteomic Analysis of a Rat Cerebral Ischemic Injury Model after Human Cerebral Endothelial Cell Transplantation.
Choi TM, Yun M, Lee JK, Park JT, Park MS, Kim HS
Journal of Korean Neurosurgical Society 2016 Nov;59(6):544-550
Journal of Korean Neurosurgical Society 2016 Nov;59(6):544-550
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SPG7 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (RC206340, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SPG7 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SPG7 mouse monoclonal antibody (TA504424) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7(RC206340).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-SPG7 mouse monoclonal antibody (TA504424).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-SPG7 antibody(TA504424),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
