27801-1-AP150UL

antibody from Invitrogen Antibodies

Targeting: SPG7 CAR, CMAR, SPG5C

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

27801-1-AP150UL

Provider

Invitrogen Antibodies

Product name

SPG7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.55 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates (HeLa, HepG2, LNCaP) were subjected to SDS PAGE followed by western blot with (Product # 27801-1-AP150UL) (SPG7 antibody) at dilution of 1:1,000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using (Product # 27801-1-AP150UL) (SPG7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using (Product # 27801-1-AP150UL) (SPG7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SPG7 (IP:(Product # 27801-1-AP150UL), 4 µg; Detection:(Product # 27801-1-AP150UL) 1:500) with HeLa cells lysate 2800 ug.