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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 16552-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMPDL3B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MRLLAWLIF LANWGGARAE PGKFWHIADL HLDPDYKVSK DPFQVCPSAG SQPVPDAGPW GDYLCDSPWA LINSSIYAMK EIEPEPDFIL WTGDDTPHVP DEKLGEAAVL EIVERLTKLI REVFPDTKVY AALGNHDFHP KNQFPAGSNN IYNQIAELWK PWLSNESIAL FKKGAFYCEK LPGPSGAGRI VVLNTNLYYT SNALTADMAD PGQQFQWLED VLTDASKAGD MVYIVGHVPP GFFEKTQNKA WFREGFNEKY LKVVRKHHRV IAGQFFGHHH TDSFRMLYDD AGVPISAMFI TPGVTPWKTT LPGVVNGANN PAIRVFEYDR ATLSLKV (1-336 aa encoded by BC014444)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.2 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 16552-1-AP (SMPDL3B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 16552-1-AP ( SMPDL3B Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 16552-1-AP (SMPDL3B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed COLO 320 cells using 16552-1-AP (SMPDL3B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 16552-1-AP ( SMPDL3B Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 16552-1-AP ( SMPDL3B Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
